Genetic variants of the bone morphogenetic protein gene and its association with estrogen and progesterone levels with litter size in Awassi ewes

This research aimed to assess the genetic polymorphism of BMP15 gene concerning sex hormone levels and birth type in Awassi ewes. The genome DNA 138 ewes was isolated (52 produced a singleton 86 twins). exon-2 amplifiable by polymerase chain reaction (PCR), so two genotypes were identified based on 141 bp amplicons: TT TA. A sequencing revealed novel mutation, c.50980646T>A, TA genotype. single nucleotide (SNP) showed high association (P≤0.01) with litter size, sheep containing this SNP had higher hormones larger sizes than without it. Ewes genotype 1.89 size their counterparts. Logistic regression confirmed that c.50980646T>A increased size. In conclusion, appears be significantly related reproductive traits (especially size) sheep. With these results, mutations are suitable for developing marker-assisted selection programs increase